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low toxicity silicone adhesive  (World Precision Instruments)


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    World Precision Instruments low toxicity silicone adhesive
    Low Toxicity Silicone Adhesive, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 98/100, based on 1559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low toxicity silicone adhesive/product/World Precision Instruments
    Average 98 stars, based on 1559 article reviews
    low toxicity silicone adhesive - by Bioz Stars, 2026-03
    98/100 stars

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    KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
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    sil  (World Precision Instruments)
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    KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
    Sil, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sil/product/World Precision Instruments
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    KLF15 knockdown canceled the effect of SIL on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) and/or KT5823 (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

    Article Title: Sildenafil suppresses the activation of hypertrophic scar fibroblasts by promoting the KLF15-mediated inhibition of LOXL1 transcription

    doi: 10.4196/kjpp.25.179

    Figure Lengend Snippet: KLF15 knockdown canceled the effect of SIL on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) and/or KT5823 (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: The cells were treated with 5 ng/ml TGF-β1 (100-21C; Peprotech) for 24 h. According to experimental groups, cells were co-treated with 10, 20, 50, and 100 μM SIL (HY-15025; MedChemExpress) and/or 1 μM KT5823 (10010965; Cayman).

    Techniques: Knockdown, Migration, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Incubation, shRNA, CCK-8 Assay, Derivative Assay, Cell Counting